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trap staining solution  (Servicebio Inc)

 
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    Structured Review

    Servicebio Inc trap staining solution
    pBTO inhibited osteoclastogenesis in vitro. (A) <t>TRAP</t> staining <t>in</t> <t>RANKL-treated</t> RAW264.7 cells stimulated with BTO and pBTO. (B) Relative mRNA levels of genes associated with osteoclastogenesis of RAW264.7 cells in the presence of RANKL, BTO or pBTO. (C) JC-1 staining in RANKL-treated RAW264.7 cells stimulated with BTO and pBTO. (D) Schematic diagram of pBTO inhibit osteoclastogenesis of macrophage (∗: p < 0.05, ∗∗: p < 0.01, ∗∗∗∗: p < 0.001).
    Trap Staining Solution, supplied by Servicebio Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/trap staining solution/product/Servicebio Inc
    Average 90 stars, based on 1 article reviews
    trap staining solution - by Bioz Stars, 2026-03
    90/100 stars

    Images

    1) Product Images from "Self-powered intracellular nanogenerator attenuates inflammatory osteolysis through mitochondrial MRS2/Mg 2+ -mediated macrophage repolarization and osteoclastogenesis inhibition"

    Article Title: Self-powered intracellular nanogenerator attenuates inflammatory osteolysis through mitochondrial MRS2/Mg 2+ -mediated macrophage repolarization and osteoclastogenesis inhibition

    Journal: Materials Today Bio

    doi: 10.1016/j.mtbio.2025.102050

    pBTO inhibited osteoclastogenesis in vitro. (A) TRAP staining in RANKL-treated RAW264.7 cells stimulated with BTO and pBTO. (B) Relative mRNA levels of genes associated with osteoclastogenesis of RAW264.7 cells in the presence of RANKL, BTO or pBTO. (C) JC-1 staining in RANKL-treated RAW264.7 cells stimulated with BTO and pBTO. (D) Schematic diagram of pBTO inhibit osteoclastogenesis of macrophage (∗: p < 0.05, ∗∗: p < 0.01, ∗∗∗∗: p < 0.001).
    Figure Legend Snippet: pBTO inhibited osteoclastogenesis in vitro. (A) TRAP staining in RANKL-treated RAW264.7 cells stimulated with BTO and pBTO. (B) Relative mRNA levels of genes associated with osteoclastogenesis of RAW264.7 cells in the presence of RANKL, BTO or pBTO. (C) JC-1 staining in RANKL-treated RAW264.7 cells stimulated with BTO and pBTO. (D) Schematic diagram of pBTO inhibit osteoclastogenesis of macrophage (∗: p < 0.05, ∗∗: p < 0.01, ∗∗∗∗: p < 0.001).

    Techniques Used: In Vitro, Staining

    pBTO improved inflammatory bone loss by modulating macrophage polarization and osteoclastogenesis in vivo. (A) Designed an mice inflammatory bone loss model. (B) Micro-CT scanning and 3D reconstruction of mice calvarial bone. pBTO inhibited LPS-induced inflammatory bone loss. (C) CD68 + Arg-1 + macrophages (arrow) in calvarial bone matrix under confocal microscope. PBTO increased the number of M2 macrophages. (D) CD68 + iNOS + macrophages (arrow) in calvarial bone matrix under confocal microscope. pBTO decreased the number of LPS-induced M1 macrophages. (E) HE staining of mice calvarial bone showed pBTO decreased LPS-induced inflammatory cell infiltration and bone resorption. (F) TRAP staining of mice calvarial bone showed pBTO decreased the number of osteoclasts.
    Figure Legend Snippet: pBTO improved inflammatory bone loss by modulating macrophage polarization and osteoclastogenesis in vivo. (A) Designed an mice inflammatory bone loss model. (B) Micro-CT scanning and 3D reconstruction of mice calvarial bone. pBTO inhibited LPS-induced inflammatory bone loss. (C) CD68 + Arg-1 + macrophages (arrow) in calvarial bone matrix under confocal microscope. PBTO increased the number of M2 macrophages. (D) CD68 + iNOS + macrophages (arrow) in calvarial bone matrix under confocal microscope. pBTO decreased the number of LPS-induced M1 macrophages. (E) HE staining of mice calvarial bone showed pBTO decreased LPS-induced inflammatory cell infiltration and bone resorption. (F) TRAP staining of mice calvarial bone showed pBTO decreased the number of osteoclasts.

    Techniques Used: In Vivo, Micro-CT, Microscopy, Staining



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    pBTO inhibited osteoclastogenesis in vitro. (A) <t>TRAP</t> staining <t>in</t> <t>RANKL-treated</t> RAW264.7 cells stimulated with BTO and pBTO. (B) Relative mRNA levels of genes associated with osteoclastogenesis of RAW264.7 cells in the presence of RANKL, BTO or pBTO. (C) JC-1 staining in RANKL-treated RAW264.7 cells stimulated with BTO and pBTO. (D) Schematic diagram of pBTO inhibit osteoclastogenesis of macrophage (∗: p < 0.05, ∗∗: p < 0.01, ∗∗∗∗: p < 0.001).
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    pBTO inhibited osteoclastogenesis in vitro. (A) <t>TRAP</t> staining <t>in</t> <t>RANKL-treated</t> RAW264.7 cells stimulated with BTO and pBTO. (B) Relative mRNA levels of genes associated with osteoclastogenesis of RAW264.7 cells in the presence of RANKL, BTO or pBTO. (C) JC-1 staining in RANKL-treated RAW264.7 cells stimulated with BTO and pBTO. (D) Schematic diagram of pBTO inhibit osteoclastogenesis of macrophage (∗: p < 0.05, ∗∗: p < 0.01, ∗∗∗∗: p < 0.001).
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    pBTO inhibited osteoclastogenesis in vitro. (A) <t>TRAP</t> staining <t>in</t> <t>RANKL-treated</t> RAW264.7 cells stimulated with BTO and pBTO. (B) Relative mRNA levels of genes associated with osteoclastogenesis of RAW264.7 cells in the presence of RANKL, BTO or pBTO. (C) JC-1 staining in RANKL-treated RAW264.7 cells stimulated with BTO and pBTO. (D) Schematic diagram of pBTO inhibit osteoclastogenesis of macrophage (∗: p < 0.05, ∗∗: p < 0.01, ∗∗∗∗: p < 0.001).
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    pBTO inhibited osteoclastogenesis in vitro. (A) <t>TRAP</t> staining <t>in</t> <t>RANKL-treated</t> RAW264.7 cells stimulated with BTO and pBTO. (B) Relative mRNA levels of genes associated with osteoclastogenesis of RAW264.7 cells in the presence of RANKL, BTO or pBTO. (C) JC-1 staining in RANKL-treated RAW264.7 cells stimulated with BTO and pBTO. (D) Schematic diagram of pBTO inhibit osteoclastogenesis of macrophage (∗: p < 0.05, ∗∗: p < 0.01, ∗∗∗∗: p < 0.001).
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    pBTO inhibited osteoclastogenesis in vitro. (A) <t>TRAP</t> staining <t>in</t> <t>RANKL-treated</t> RAW264.7 cells stimulated with BTO and pBTO. (B) Relative mRNA levels of genes associated with osteoclastogenesis of RAW264.7 cells in the presence of RANKL, BTO or pBTO. (C) JC-1 staining in RANKL-treated RAW264.7 cells stimulated with BTO and pBTO. (D) Schematic diagram of pBTO inhibit osteoclastogenesis of macrophage (∗: p < 0.05, ∗∗: p < 0.01, ∗∗∗∗: p < 0.001).
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    Image Search Results


    pBTO inhibited osteoclastogenesis in vitro. (A) TRAP staining in RANKL-treated RAW264.7 cells stimulated with BTO and pBTO. (B) Relative mRNA levels of genes associated with osteoclastogenesis of RAW264.7 cells in the presence of RANKL, BTO or pBTO. (C) JC-1 staining in RANKL-treated RAW264.7 cells stimulated with BTO and pBTO. (D) Schematic diagram of pBTO inhibit osteoclastogenesis of macrophage (∗: p < 0.05, ∗∗: p < 0.01, ∗∗∗∗: p < 0.001).

    Journal: Materials Today Bio

    Article Title: Self-powered intracellular nanogenerator attenuates inflammatory osteolysis through mitochondrial MRS2/Mg 2+ -mediated macrophage repolarization and osteoclastogenesis inhibition

    doi: 10.1016/j.mtbio.2025.102050

    Figure Lengend Snippet: pBTO inhibited osteoclastogenesis in vitro. (A) TRAP staining in RANKL-treated RAW264.7 cells stimulated with BTO and pBTO. (B) Relative mRNA levels of genes associated with osteoclastogenesis of RAW264.7 cells in the presence of RANKL, BTO or pBTO. (C) JC-1 staining in RANKL-treated RAW264.7 cells stimulated with BTO and pBTO. (D) Schematic diagram of pBTO inhibit osteoclastogenesis of macrophage (∗: p < 0.05, ∗∗: p < 0.01, ∗∗∗∗: p < 0.001).

    Article Snippet: Based on our previous research [ ], RAW 264.7 cells were incubated with 100 μg/mL BTO and pBTO, and stimulated with 50 ng/mL RANKL (peprotech, USA) for 3–5 days and then subjected to TRAP staining (Servicebio, China).

    Techniques: In Vitro, Staining

    pBTO improved inflammatory bone loss by modulating macrophage polarization and osteoclastogenesis in vivo. (A) Designed an mice inflammatory bone loss model. (B) Micro-CT scanning and 3D reconstruction of mice calvarial bone. pBTO inhibited LPS-induced inflammatory bone loss. (C) CD68 + Arg-1 + macrophages (arrow) in calvarial bone matrix under confocal microscope. PBTO increased the number of M2 macrophages. (D) CD68 + iNOS + macrophages (arrow) in calvarial bone matrix under confocal microscope. pBTO decreased the number of LPS-induced M1 macrophages. (E) HE staining of mice calvarial bone showed pBTO decreased LPS-induced inflammatory cell infiltration and bone resorption. (F) TRAP staining of mice calvarial bone showed pBTO decreased the number of osteoclasts.

    Journal: Materials Today Bio

    Article Title: Self-powered intracellular nanogenerator attenuates inflammatory osteolysis through mitochondrial MRS2/Mg 2+ -mediated macrophage repolarization and osteoclastogenesis inhibition

    doi: 10.1016/j.mtbio.2025.102050

    Figure Lengend Snippet: pBTO improved inflammatory bone loss by modulating macrophage polarization and osteoclastogenesis in vivo. (A) Designed an mice inflammatory bone loss model. (B) Micro-CT scanning and 3D reconstruction of mice calvarial bone. pBTO inhibited LPS-induced inflammatory bone loss. (C) CD68 + Arg-1 + macrophages (arrow) in calvarial bone matrix under confocal microscope. PBTO increased the number of M2 macrophages. (D) CD68 + iNOS + macrophages (arrow) in calvarial bone matrix under confocal microscope. pBTO decreased the number of LPS-induced M1 macrophages. (E) HE staining of mice calvarial bone showed pBTO decreased LPS-induced inflammatory cell infiltration and bone resorption. (F) TRAP staining of mice calvarial bone showed pBTO decreased the number of osteoclasts.

    Article Snippet: Based on our previous research [ ], RAW 264.7 cells were incubated with 100 μg/mL BTO and pBTO, and stimulated with 50 ng/mL RANKL (peprotech, USA) for 3–5 days and then subjected to TRAP staining (Servicebio, China).

    Techniques: In Vivo, Micro-CT, Microscopy, Staining